Conformational variation of proteins at room temperature is not dominated by radiation damage

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Conformational variation of proteins at room temperature is not dominated by radiation damage

Protein crystallography data collection at synchrotrons is routinely carried out at cryogenic temperatures to mitigate radiation damage. Although damage still takes place at 100 K and below, the immobilization of free radicals increases the lifetime of the crystals by approximately 100-fold. Recent studies have shown that flash-cooling decreases the heterogeneity of the conformational ensemble ...

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ژورنال

عنوان ژورنال: Journal of Synchrotron Radiation

سال: 2017

ISSN: 1600-5775

DOI: 10.1107/s1600577516017343